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Name of the University : Pondicherry University
Name of the Exam : BINC Bioinformatics National Certification Examination
Subject: Paper – II
Document Type : Sample paper
Year : 2015
Website :
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BINC Examination Paper – II Question :

Note :
** In Paper II of BINC – 2015, Candidates have to answer 20 questions out of a total of 55 questions divided into three sections as below.
** Each question will carry 10 marks.

Related : Pondicherry University BINC Exam Paper – I Questions :

Section A: Bioinformatics (Answer any 10 out of 25 questions)
Section B: Biology, Chemistry & Physics (Answer any 5 out of 15 questions)
Section C: Mathematics, Statistics & IT (Answer any 5 out of 15 questions)

Section – A :
1. a) What do you understand by sensitivity and specificity in BLAST? (3)
b) Suppose in a BLAST search you got an E-value of about 2 x 10 -10. What does this E value mean? Name two parameters that determine the E-value. (4)
c) Briefly explain the major differences between BLAST method and PSI-BLAST Method. (3)

2. a) What is dynamic programming algorithm? (2)
b) What is the basic difference between Needleman-Wunsch and Smith-Waterman algorithm? (4)
c) What are BLAST and FASTA? (4)

3. a) State the database structure of Gen Bank and how it can be used to make discoveries.
b) Give the importance of OMIM andSNP database (DbSNP). Describe briefly these databases. (5+5)

4. a) Give examples of protein- protein interaction databases and describe their utility in understanding protein- protein interactions. (6)
b) How does species 2000 databases different from ICTV database? (4)

Section – B :
5. a) Golgi generates two types of vesicles that are destined for lysosomes and plasma membrane. In what way the two types of vesicles differ from each other?
b) Describe the mechanism by which these vesicles find and fuse with the right target. (4+6)

6. If Down’s syndrome occurs in 1 out of 700 births and Turner’s syndrome in 1 out of 5000 cases, answer the following:
I. What are the chances that two cases of Down’s syndrome occur in one hospital on the same day? (3)
II. If the number of births in a country is 3,50,00,000, how many cases of Down’s Syndromes are likely to be recorded among the new born? (3)
III. If Down’s syndrome and Turner syndrome are randomly distributed, what are the chances that the newborn will be found with both syndromes? (4)

7. a) Draw a peptide group and show where water molecules can be bound non-covalently (4)
b) Between the two molecules, water and carbon dioxide, which one has a dipole moment? Is carbon-dioxide IR active? Give reason. (4)
c) Between electrostatic and van der Waals energies, which effect will be felt at longer distances? (2)

8. a) If the pKa of the ? amino group in lysine is 9.2, what will be the percentage of the lysine that will have a ? protonated amino group at a pH of 7.0 (4)

b) A molecule shows an absorbance of 1.00. The concentration of the solution is 2 X 10-5 ML-1. The measurement was done in a cuvette of length 2 cm. (4)
1) What is the molar absorptivity?
2) What is the percentage of light that reaches the detector?

c) The activation energy for the hydrolysis of sucrose is 107 kJmol-1 in the presence of H+. When the enzyme saccharase is added, the activation energy is 36 kJmol-1. What is the order of magnitude change in the rate of the reaction of the enzyme catalysed to the H+ catalysed reaction? (2)

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