kakatiya.ac.in M.Sc Microbiology Practical Exam Paper Model : University

Name of the College : Kakatiya University
Degree : M.Sc.
Name Of The Subject : Microbiology
Document type : Model Question Paper

Website : kakatiya.ac.in
Download Model Question Papers : https://www.pdfquestion.in/uploads/7511-M.ScMicrobiology.pdf

M.Sc. Microbiology Practical Examination Paper :

1. Major Experiment 20 Marks
a) Principle & procedure 5
b) Conducting experiment 10
c) Interpretation of results & conclusions 5

Related : Baba Farid University of Health Sciences BDS General Pathology & Microbiology Question Paper : www.pdfquestion.in/13231.html

2. Major Experiment 20 Marks
3 Minor Experiment 10 Marks
a) Principle & procedure 3
b) Conducting the experiment 5
c) Interpretation of results & conclusions 2
4 Minor Experiment 10 Marks
5. Spotting (4 Nos) (4×5=20) 20 Marks
a) Identification 2
b) Critical notes 3
6. Record 10 Marks
7 Seminar / Internal 10 Marks
(To be submitted by the in charge of the department duly signed by Principal)

Practical Paper- I :
Virology :
1. Preparation of different types of media
2. Isolation and enumeration of bacterial and fungal population in air.
3. Enumeration of bacterial population in water.
4. Isolation and enumeration of bacterial and fungal population in soil
5. Demonstration of bacterial motility by Hanging drop technique
6. Staining techniques.
i) Gram staining ii) Cell wall staining iii) Endospore staining
iv) Flagella staining v) Capsule staining vi) Staining of PHB granules
vii) Staining of phosphate granules
7. IMVIC tests ( Inole, methylred, Voges prausker and citrate test )
8. Oxidast test
9. Carbohydrate fermentation & Gas production
10. Catalase test
11. Gelatinase test
12. Caseinase test
13. Amylase test
14. H 2 S production test
15. Nitrate reduction test
16. Litmus milk reactions
17. Urease test
18. Determination of bacterial growth by turbidometric method
19, Effect of temperature on bacterial growth.
20. Effect of osmotic pressure on bacterial growth.
21. Effect of p H on bacterial growth
22. Cultivation of anaerobes
i) Shake culture technique ii) Pyrogallic acid method iii) Anaerobic gas pack
23. Growth kinetics (problems)
24 Estimation of chlorophyll in healthy and viral diseased plants
25 Study of symptomology of plant, animal and human diseases caused by viruses.
26 Estimation of proteins in healthy and viral diseased plants
27 Estimation of DNA in healthy and viral diseased plants
28 Estimation of RNA in healthy and viral diseased plants
29 Transmission of viruses by grafting
30 Transmission of viruses by aphids
31 Sap transmission of plant viruses
32 Isolation of phages from sewage
33 Propagation of animal viruses in embryonated eggs
a) Amniotic cavity b) Chorioallantoic cavity c) Yolk sac
34 Preparation of bacteriophage stocks
35 One step growth curve experiments.
36 Problems on i) Phage enumeration ii) Acid end point iii) Hemagglutination assay
37 Micrometry-measure the fungal spore dimensions by using ocular and stage micrometers and calculation of the mean and standard deviation
38 Demonstration of mycorrhizal association
39 Identification of fungal cultures, algal cultures, and Protozoa
40 Electron photo micrographic study of virus

Practical Examination :
1. Major Experiment : 20 Marks
1. Micrometry-measurement of the fungal spore dimensions by using ocular and stage micrometers and calculation of the mean and standard deviation
2. Demonstration of mycorrhiza association
3. Demonstration of bacterial motility by hanging drop technique
4. Gram staining
5. Cell wall staining
6. Endospore staining
7. Flagella staining
8. Capsule staining
9. Staining of PHB granules
10. Staining of phosphate granules
11. Measurement of bacterial growth by turbidometric method

2. Major Experiment : 20 Marks
1. Estimation of chlorophyll in healthy and viral diseased plants
2. Study of symptomology of plant, animal and human diseases caused by viruses.
3. Estimation of proteins in healthy and viral diseased plants
4. Estimation of DNA in healthy and viral diseased plants
5. Estimation of RNA in healthy and viral diseased plants
6. Transmission of plant viruses by grafting
7. Transmission of plant viruses by aphids
8. Sap transmission of plant viruses
9. Isolation of coliphages from sewage

3. Minor Experiment : 10 Marks
1. Indole test
2. Methyl red test
3. Voges Proskauer test
4. Citrate test
5. Oxidase test
6. Carbohydrate fermentation & Gas production
7. Catalase test
8. Gelatinase test
9. Caseinase test
10. Amylase test
11. H 2 S production test
12. Nitrate reduction test
13. Litmus milk reactions
14. Urease test
15. Growth kinetics (problems)
16. Effect of osmotic pressure on bacterial growth.
17. Effect of p H on bacterial growth