Fundamental Methods in Microbiology B.Sc Question Bank : nmu.ac.in
Name of the University : North Maharashtra University
Degree : B.Sc
Department : Microbiology
Year : I
Name Of The Subject :Fundamental Methods in Microbiology
Document type : Question Bank
Website : nmu.ac.in
Download Model/Sample Question Papers : https://www.pdfquestion.in/uploads/nmu.ac.in/5296.-F.%20Y.%20B.%20Sc.(Microbiology)%20Paper-II%20Question%20Bank.pdf
NMU Microbiology Model Paper
Paper II : Fundamental Methods in Microbiology
Chapter 1 :
Microscopy
Q. 12 marks each
1. What is aberration- Explain different types of aberrations.
Related : North Maharashtra University Algebra And Trignometry B.Sc Question Bank : www.pdfquestion.in/5294.html
2. What is microscope- Explain different types of lenses used in compound
3. What is numerical aperture- Explain the principle and working of oil immersion
4. Compare bright field and dark field microscope. Add a note on image formation
5. Explain the concepts of magnification, resolution and numerical aperture.
Q. Short notes – 4 marks each.
1. Compare bright field and dark field microscope.
2. Types and functions of condenser.
3. Types and functions of objective.
4. Types and functions of ocular.
5. How is image formed in compound microscope-
6. Explain the principle and working of oil immersion lens.
7. Magnification.
8. Resolution.
9. Numerical aperture.
10. Chromatic aberration.
Q. Short answer – 6 marks each.
1. Define microscope. Explain types and functions of condenser.
2. Define microscope. Explain types and functions of objective.
3. Define aberration. Explain chromatic and spherical aberration.
4. Define aberration. Explain coma, astigmatism and distortion.
5. With suitable diagram explain image formation in compound microscope.
6. With suitable diagram explain the principle and working of oil immersion lens.
7. What is microscope- Compare bright field and dark field microscope.
8. Explain the terms magnification and resolution.
9. What is aberration- Explain any 3 types.
10. Numerical aperture.
Chapter 2 :
Biological staining
Q. 12 marks each
1. What is dye and stain- Explain the chromophore and auxochrome concept with
2. Define dye and explain different types of dyes with suitable example.
3. What is dye and stain- Explain the uses of stains.
4. What is mordant- How it is classified- Give mechanism of action with suitable
5. What is mordant- Explain the mechanism of action and method of application.
6. What is stain- Explain simple staining.
7. What is stain- Explain Gram staining.
8. What is stain- Explain Acid fast staining.
Q. Short notes – 4 marks each.
1. Concept of dye and stain.
2. Explain the term chromophore.
3. Explain the term auxochrome.
4. Explain the uses of stain.
5. Neutral dyes.
6. What is mordant- Explain the method of application.
7. What is mordant- Give the mechanism of action.
8. What is mordant- Explain the types and examples.
9. Explain the terms natural, synthetic and leuco dyes.
10. Comment on Gram variable reaction.
11. Write a note on differences between Gram positive and Gram negative cell
12. Explain the mechanism of Gram staining.
13. Explain the mechanism of acid fast staining.
14. What is monochrome staining- Give procedure.
15. Give examples of stains used in simple and differential staining
Q. Short answer – 6 marks each :
1. What is dye? Explain acidic, basic and neutral dyes with examples.
2. What is dye? Explain natural, synthetic and leuco dyes with examples.
3. Explain the types and mechanism of action of mordant.
4. Explain the mechanism of action of mordant and give examples.
5. Write a note on simple staining.
6. Write a note on Gram staining.
7. Write a note on acid fast staining.
Q. Definition —- 2 marks each :
1. Stain.
2. Dye.
3. Chromophore.
4. Auxochrome.
5. Mordant.
6. Leuco dyes.
7. Synthetic dyes.
8. Compound dyes.
9. Acidic dyes.
10. Basic dyes.
11. Simple staining.
12. Differential staining.
Cultivation of Microbes
Chapter 3 :
Q. 12 marks each
1. Explain the nutritional requirements of bacteria.
2. Define medium. Write a note on ingredients used in preparation of laboratory media.
3. What is medium? Explain types of media based on application.
4. What is medium? Explain types of media based on nature of ingredients.
5. What is enrichment? Explain physical methods of enrichment.
6. What is enrichment? Explain chemical and biological methods of enrichment.
7. Explain MacIntosh and Brewer’s jar.
8. Explain chick embryo technique.
9. Explain use of cell cultures in cultivation of animal viruses.
Q. Short notes – 4 marks each. :
1. Comment on carbon, nitrogen and energy requirement of bacteria.
2. Define medium. What is the role of peptone, NaCl and agar in the medium?
3. Define medium. What is the role of yeast extract, malt extract and agar in the medium?
4. Define medium. Explain the types of medium based on physical state.
5. Explain synthetic and semi synthetic medium with example.
6. What is enrichment? Explain use of incubation temperature and pH of medium for enrichment.
7. What is enrichment? Explain use of inhibitory substances for enrichment.
8. What is enrichment? Explain micromanipulator technique.
9. Define anaerobe. Explain candle jar method.
10. Write a note on MacIntosh jar.
11. Define anaerobe. Explain use of pyrogallolic acid.
12. Comment on cultivation of plant viruses.
13. Types of tissue culture.
Q. Short answer – 6 marks each. :
1. What is cultivation? Explain growth factor requirements of bacteria.
2. What is cultivation? Explain carbon, nitrogen and energy requirements of bacteria.
3. What is cultivation? Explain H,S,P and mineral requirements of bacteria.