Organisation : Central Board of Secondary Education (CBSE)
Class Name : 12th/HSC (Class XII)
Subject : Biotechnology
Download : 2025 Exam Question Paper
Website : https://www.cbse.gov.in/cbsenew/question-paper.html
CBSE Class XII Biotechnology Question Paper
Central Board of Secondary Education (CBSE) Class XII Biotechnology 2025 Exam Question Paper.
Related / Similar Question Paper : CBSE Class XII Beauty & Wellness Question Paper 2025
CBSE Class XII Biotechnology Questions
Section – A:
1. The DNA ligase enzyme that is frequently used to ligate different DNA fragments in order to generate rDNA molecules is isolated from :
(A) T2 Bacteriophage
(B) T4 Bacteriophage
(C) Lambda Bacteriophage
(D) M13 filamentous phage
2. Protein Efficiency Ratio (PER) is used as a measure of growth expressed in terms of :
(A) Weight gain of an adult by consuming 1 g of food protein.
(B) Weight gain of an adult by consuming 100 g of food protein.
(C) Protein nitrogen that is retained by the body by consuming 1 g of food protein.
(D) Protein nitrogen that is retained by the body by consuming 100 g of food protein.
3. At the end of one cycle of PCR, two DNA molecules will become _______.
(A) Eight
(B) Sixteen
(C) Four
(D) Two
4. Single base difference in ApoE gene is associated with :
(A) Resistance to HIV (Human Immunodeficiency Virus)
(B) Migraine
(C) Huntington disease
(D) Alzheimer’s disease
5. One of the carbon source used in microbial cell culture is :
(A) Olive oil
(B) Cereal grains
(C) Ammonium salts
(D) Growth factors
6. A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result ? 1
(A) M13 based vector
(B) Bacteriophage Lambda based vector
(C) Plasmid cloning vector of pUC family
(D) Plasmid vector pBR 322
7. Absence of the enzyme adenosine deaminase in humans causes :
(A) Thallasemia
(B) Sickle cell anaemia
(C) SCID
(D) Mad cow disease
8. The technique to isolate and grow human embryonic stem cells in culture was developed by:
(A) Ceasar Milstein
(B) James Thomson
(C) George Kohler
(D) George Gay
9. Gene prediction for bacterial genomes can be done by using the computer program _______.
(A) GeneMark
(B) GENSCAN
(C) UniProtKB
(D) RefSeq
10. _______ can be categorized as a food source of nutraceutical proteins.
(A) Seed storage proteins
(B) Soya protein
(C) Milk proteins
(D) Whey protein concentrates
11. Identification of disease specific proteins can be done by using the approach of :
(A) Functional proteomics
(B) Structural proteomics
(C) Expression proteomics
(D) Proteome mining
12. Herceptin is a monoclonal antibody approved for :
(A) Therapy of early stage breast cancer.
(B) Reversal of acute rejection of transplanted organs.
(C) Stimulation of erythropoiesis.
(D) Use in certain patients having a heart attack
For Questions 13 to 16, two statements are given – one labelled Assertion (A) and other labelled Reason (R). Select the correct answer to these questions from the codes (A), (B), (C) and (D) as given below :
(A) Both Assertion (A) and Reason (R) are true and the Reason (R) is the correct explanation of the Assertion (A).
(B) Both Assertion (A) and Reason (R) are true, but the Reason (R) is not the correct explanation of the Assertion (A).
(C) Assertion (A) is true, but Reason (R) is false.
(D) Assertion (A) is false, but Reason (R) is true.
13. Assertion (A) : CO 2 incubators prevent the desiccation of the animal cell culture medium.
Reason (R) : A pan of water is kept at all times in a CO 2 incubator chamber to maintain high relative humidity.
14. Assertion (A) : Contact inhibition is absent in cancer cells.
Reason (R) : Normal cells stop growing when they reach the walls of the container.
15. Assertion (A) : Proteins have diverse functions.
Reason (R) : All proteins are enzymes.
16. Assertion (A) : One of the first attempts to study the molecular basis of sickle cell anaemia was to compare the electrophoretic mobility of normal haemoglobin (Hb) and sickle cell haemoglobin (scHb).
Reason (R) : Sickle cell haemoglobin (scHb) moved faster than normal haemoglobin (Hb) in electrophoresis.
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